Background:
@EN The purpose of this study is to investigate diabetic changes of both pancreas and kidney in NOD mice which are animal model for type 1 diabetes, and to develop animal model for human diabetic nephropathy more early and higher incidence than
NOD
mice.
@ES Methods:
@EN The 8-week-old NOD and ICR mice were injected with cyclophosphamide intraperitoneally at 200㎎/㎏body weight Blood glucose concentration and body weight are measured every week, and diabetic animals were determined over 200㎎/dl of blood
glucose
level. Light microscopical, immunohistochemical, electron microscopical studies were carried out in both pancreas and kidney of NOD mice.
@ES Results :
@EN 1) Diabetes were significantly promoted in cyclophosphamide-induced NOD mice(90%) compared with those of NOD mice (60%).
2) The NOD and NOD+CY mice showed markedly decreased number of B cells, but no different changes of A cells. These mice also showed glomerular hypertrophy, proliferation of mesangium.
3) The nephropathy in the CY-induced NOD mouse was strongly correlated with the insulitis in the same animal.
4) Electron microscopical investigation showed partial thickness of basement membrane in NOD and NOD+CY mice. NOD+CY mice were more severely and early affected than NOD mice, moreover were shown partial fusion of pedicels of podocytes.
@ES Conclusion:
@EN As lymphocyte infiltration, decreased B cell number in pancreatic islets, followed by high blood glucose concentration and early and higher incidence diabetic nephropathy as described above, cyclophosph-amide-induced NOD mice could be used
as
model
of type 1 diabetic research.
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